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JoVE Journal Cancer Research
Generazione e imaging di organoidi epiteliali di topo e umani da tessuto mammario normale e tumorale senza passaggio
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Generazione e imaging di organoidi epiteliali di topo e umani da tessuto mammario normale e tumorale senza passaggio

Article DOI: 10.3791/64626-v • 08:57 min • November 11th, 2022
November 11th, 2022 •
1,2, 2,3,4,5, 1,2, 1,2, 1,2, 6, 1,2, 2,3,4,5, 1,2,3,4
1Department of Internal Medicine, Division of Hematology and Oncology, University of Texas Southwestern Medical Center, 2Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, 3Department of Molecular Biology, University of Texas Southwestern Medical Center, 4Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, 5Department of Cell Biology, University of Texas Southwestern Medical Center, 6Division of Medical Oncology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California

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Questo protocollo discute un approccio per generare organoidi epiteliali dal tessuto mammario primario normale e tumorale attraverso la centrifugazione differenziale. Inoltre, sono incluse istruzioni per la coltura tridimensionale e l'imaging immunofluorescente di organoidi incorporati.

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Ricerca sul cancro numero 189
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