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Journal / Cancer Research / Génération et imagerie d’organoïdes épithéliaux…
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JoVE Journal Cancer Research
Génération et imagerie d’organoïdes épithéliaux de souris et humains à partir de tissus mammaires normaux et tumoraux sans passage
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Génération et imagerie d’organoïdes épithéliaux de souris et humains à partir de tissus mammaires normaux et tumoraux sans passage

Article DOI: 10.3791/64626-v • 08:57 min • November 11th, 2022
November 11th, 2022 •
1,2, 2,3,4,5, 1,2, 1,2, 1,2, 6, 1,2, 2,3,4,5, 1,2,3,4
1Department of Internal Medicine, Division of Hematology and Oncology, University of Texas Southwestern Medical Center, 2Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, 3Department of Molecular Biology, University of Texas Southwestern Medical Center, 4Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, 5Department of Cell Biology, University of Texas Southwestern Medical Center, 6Division of Medical Oncology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California

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Ce protocole traite d’une approche pour générer des organoïdes épithéliaux à partir de tissu mammaire primaire normal et tumoral par centrifugation différentielle. En outre, des instructions sont incluses pour la culture tridimensionnelle ainsi que l’imagerie immunofluorescente des organoïdes intégrés.

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Recherche sur le cancer numéro 189
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