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Journal / Cancer Research / Erzeugung und Bildgebung von Epithelorganoiden…
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JoVE Journal Cancer Research
Erzeugung und Bildgebung von Epithelorganoiden von Mäusen und Menschen aus normalem und Tumor-Brustgewebe ohne Passaging
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Erzeugung und Bildgebung von Epithelorganoiden von Mäusen und Menschen aus normalem und Tumor-Brustgewebe ohne Passaging

Article DOI: 10.3791/64626-v • 08:57 min • November 11th, 2022
November 11th, 2022 •
1,2, 2,3,4,5, 1,2, 1,2, 1,2, 6, 1,2, 2,3,4,5, 1,2,3,4
1Department of Internal Medicine, Division of Hematology and Oncology, University of Texas Southwestern Medical Center, 2Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, 3Department of Molecular Biology, University of Texas Southwestern Medical Center, 4Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, 5Department of Cell Biology, University of Texas Southwestern Medical Center, 6Division of Medical Oncology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California

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In diesem Protokoll wird ein Ansatz zur Erzeugung von Epithelorganoiden aus primärem Normal- und Tumorbrustgewebe durch differentielle Zentrifugation erörtert. Darüber hinaus sind Anleitungen für die dreidimensionale Kultivierung sowie die Immunfluoreszenz-Bildgebung von eingebetteten Organoiden enthalten.

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Krebsforschung Heft 189
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