Generating Human Gastric Organoids from Upper Endoscopy Biopsies

January 26th, 2024

Generation of and Comparison Between Patient-Derived Gastric Organoids from Different Regions of the Stomach (Video) | JoVE

01:22 min

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Isolating Single Cells from Upper Endoscopy Biopsies Derived from Gastric Patients for Organoid Generation (Video) | JoVE

02:18 min

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Embedding Gastric Biopsy-Derived Single Cells in a Basement Membrane Matrix Dome for Organoid Generation (Video) | JoVE

02:03 min

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Routine Passaging of Patient-Derived Gastric Organoids Through Fragmentation (Video) | JoVE

03:08 min

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Kole H. Buckley¹, Keely A. Beyries¹, Sandra Ryeom², Sam S. Yoon², Bryson W. Katona¹

¹Division of Gastroenterology and Hepatology, University of Pennsylvania Perelman School of Medicine, ²Department of Surgery, Columbia University Irving Medical Center

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Gastrisk pasientavledede organoider finner økende bruk i forskning, men formelle protokoller for generering av humane gastriske organoider fra enkeltcellefordøyelser med standardisert såtetthet mangler. Denne protokollen presenterer en detaljert metode for pålitelig å lage mageorganoider fra biopsivev oppnådd under øvre endoskopi.

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Generating Human Gastric Organoids from Upper Endoscopy Biopsies

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