Generating Human Gastric Organoids from Upper Endoscopy Biopsies

January 26th, 2024

Generation of and Comparison Between Patient-Derived Gastric Organoids from Different Regions of the Stomach (Video) | JoVE

01:22 min

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Isolating Single Cells from Upper Endoscopy Biopsies Derived from Gastric Patients for Organoid Generation (Video) | JoVE

02:18 min

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Embedding Gastric Biopsy-Derived Single Cells in a Basement Membrane Matrix Dome for Organoid Generation (Video) | JoVE

02:03 min

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Routine Passaging of Patient-Derived Gastric Organoids Through Fragmentation (Video) | JoVE

03:08 min

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Kole H. Buckley¹, Keely A. Beyries¹, Sandra Ryeom², Sam S. Yoon², Bryson W. Katona¹

¹Division of Gastroenterology and Hepatology, University of Pennsylvania Perelman School of Medicine, ²Department of Surgery, Columbia University Irving Medical Center

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Gastrisk patientafledte organoider finder stigende anvendelse i forskning, men formelle protokoller til generering af humane gastriske organoider fra enkeltcellefordøjelser med standardiseret såtæthed mangler. Denne protokol præsenterer en detaljeret metode til pålideligt at skabe gastriske organoider fra biopsivæv opnået under øvre endoskopi.

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Generating Human Gastric Organoids from Upper Endoscopy Biopsies

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