Generation of and Comparison Between Patient-Derived Gastric Organoids from Different Regions of the Stomach (Video) | JoVE

Kole H. Buckley; Keely A. Beyries; Sandra Ryeom; Sam S. Yoon; Bryson W. Katona

doi:10.3791/66094

Generating Human Gastric Organoids from Upper Endoscopy Biopsies

January 26th, 2024

Generation of and Comparison Between Patient-Derived Gastric Organoids from Different Regions of the Stomach (Video) | JoVE

01:22 min

•

Isolating Single Cells from Upper Endoscopy Biopsies Derived from Gastric Patients for Organoid Generation (Video) | JoVE

02:18 min

•

Embedding Gastric Biopsy-Derived Single Cells in a Basement Membrane Matrix Dome for Organoid Generation (Video) | JoVE

02:03 min

•

Routine Passaging of Patient-Derived Gastric Organoids Through Fragmentation (Video) | JoVE

03:08 min

•

Kole H. Buckley¹, Keely A. Beyries¹, Sandra Ryeom², Sam S. Yoon², Bryson W. Katona¹

¹Division of Gastroenterology and Hepatology, University of Pennsylvania Perelman School of Medicine, ²Department of Surgery, Columbia University Irving Medical Center

Summary
Automatic Translation

العربية (Arabic)
中文 (Chinese)
dansk (Danish)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
हिंदी (Hindi)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
norsk (Norwegian)
português (Portugese)
русский (Russian)
español (Spanish)
svenska (Swedish)
Türkçe (Turkish)

Gastric patient-derived organoids find increasing use in research, yet formal protocols for generating human gastric organoids from single-cell digests with standardized seeding density are lacking. This protocol presents a detailed method for reliably creating gastric organoids from biopsy tissue obtained during upper endoscopy.

Article

Generating Human Gastric Organoids from Upper Endoscopy Biopsies

Get cutting-edge science videos from JoVE sent straight to your inbox every month.