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JoVE Journal Cancer Research
Icke-destruktiv utvärdering av regional celltäthet inom tumöraggregat efter läkemedelsbehandling
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Icke-destruktiv utvärdering av regional celltäthet inom tumöraggregat efter läkemedelsbehandling

Article DOI: 10.3791/64030-v • 10:13 min • June 21st, 2022
June 21st, 2022 •
1, 2, 2, 1
1Rensselaer Polytechnic Institute, 2Albany Medical College

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Det nuvarande protokollet utvecklar en bildbaserad teknik för snabb, icke-destruktiv och etikettfri regional celldensitets- och viabilitetsmätning inom 3D-tumöraggregat. Resultaten avslöjade en celldensitetsgradient, med högre celltäthet i kärnregioner än yttre lager vid utveckling av aggregat och övervägande perifer celldöd i HER2 + -aggregat behandlade med Trastuzumab.

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Cancerforskning nummer 184
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