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JoVE Journal Cancer Research
Ikke-destruktiv evaluering af regional celletæthed inden for tumoraggregater efter lægemiddelbehandling
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Ikke-destruktiv evaluering af regional celletæthed inden for tumoraggregater efter lægemiddelbehandling

Article DOI: 10.3791/64030-v • 10:13 min • June 21st, 2022
June 21st, 2022 •
1, 2, 2, 1
1Rensselaer Polytechnic Institute, 2Albany Medical College

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Den nuværende protokol udvikler en billedbaseret teknik til hurtig, ikke-destruktiv og etiketfri regional celletæthed og levedygtighedsmåling inden for 3D-tumoraggregater. Resultaterne afslørede en celletæthedsgradient med højere celletætheder i kerneområder end ydre lag i udviklingsaggregater og overvejende perifer celledød i HER2+ aggregater behandlet med Trastuzumab.

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Kræftforskning nummer 184
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