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JoVE Journal Biology
Funksjonsvurdering av kinesin-7 CENP-E i spermatocytter ved bruk av in vivo hemming, immunfluorescens og flowcytometri
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Funksjonsvurdering av kinesin-7 CENP-E i spermatocytter ved bruk av in vivo hemming, immunfluorescens og flowcytometri

Article DOI: 10.3791/63271-v • 09:41 min • December 28th, 2021
December 28th, 2021 •
1,2, 1,2, 3,4, 1,2, 1,2, 1,2, 1,2, 1,2
1Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, 3Fujian Obstetrics and Gynecology Hospital, 4Medical Research Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University

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Denne artikkelen rapporterer en in vivo hemming av CENP-E gjennom abdominal kirurgi og testikkelinjeksjon av GSK923295, en verdifull modell for mannlig meiotisk deling. Ved hjelp av immunfluorescens, flowcytometri og transmisjonselektronmikroskopianalyser viser vi at CENP-E-hemming resulterer i kromosomfeilstilling og genominstabilitet i musespermatocytter.

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Denne måneden i JoVE utgave 178 kinesin spermatocytt meiose CENP-E spindel kromosom mikrotubuli
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