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Transfection, Isolation, and Screening of the CENP-E Knockout HeLa Cells

Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

Article DOI: 10.3791/201002-v • 04:10 min • June 23rd, 2023
June 23rd, 2023 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 3,4, 1,2
1Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, 3College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, 4Medical Research Center, Fujian Maternity and Child Health Hospital

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Biology CENP-E Knockout Centromere-associated Protein-E Kinesin Kinetochore-microtubule Capture Chromosome Alignment Spindle Assembly Checkpoint HeLa Cells Phenotype-based Screening Chromosome Misalignment BUB1 Mitotic Checkpoint Serine/threonine Kinase B (BubR1) Mitotic Defects
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