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In situ Utforskning av Murine Megakaryopoiesis med transmissionselektronmikroskopi
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In situ Utforskning av Murine Megakaryopoiesis med transmissionselektronmikroskopi

Article DOI: 10.3791/62494-v • 08:15 min • September 8th, 2021
September 8th, 2021 •
1, 1, 1, 1
1Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS

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Här presenterar vi ett protokoll för att analysera ultrastructure av megakaryocyterna in situ med hjälp av överföring elektronmikroskopi (TEM). Murin benmärg samlas in, fixas, bäddas in i epoxiharts och skärs i ultrathin sektioner. Efter kontrastfärgning observeras benmärgen under ett TEM-mikroskop vid 120 kV.

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Biologi nummer 175 megakaryocyt trombopoies benmärg transmissionselektronmikroskopi in situ
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