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Sul posto Esplorazione della megacariopoiesi murina usando la microscopia elettronica a trasmissione
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Sul posto Esplorazione della megacariopoiesi murina usando la microscopia elettronica a trasmissione

Article DOI: 10.3791/62494-v • 08:15 min • September 8th, 2021
September 8th, 2021 •
1, 1, 1, 1
1Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS

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Qui presentiamo un protocollo per analizzare l'ultrastruttura dei megacariociti in situ utilizzando la microscopia elettronica a trasmissione (TEM). I midolli ossei murini vengono raccolti, fissati, incorporati in resina epossidica e tagliati in sezioni ultrassteste. Dopo la colorazione di contrasto, il midollo osseo viene osservato al microscopio TEM a 120 kV.

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Biologia Numero 175 megacariociti trombopoiesi midollo osseo microscopia elettronica a trasmissione in situ
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