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Quantitative Methoden zur Untersuchung der Protein-Arginin-Methyltransferase-1-9-Aktivität in Zellen
Investigating the Spreading and Toxicity of Prion-like Proteins Using the Metazoan Model Organism C. elegans
Journal (Biology)
Investigating the Spreading and Toxicity of Prion-like Proteins Using the Metazoan Model Organism C. elegans
Quick Fluorescent In Situ Hybridization Protocol for Xist RNA Combined with Immunofluorescence of Histone Modification in X-chromosome Inactivation
Journal (Biology)
Quick Fluorescent In Situ Hybridization Protocol for Xist RNA Combined with Immunofluorescence of Histone Modification in X-chromosome Inactivation
 
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Quantitative Methoden zur Untersuchung der Protein-Arginin-Methyltransferase-1-9-Aktivität in Zellen

Article DOI: 10.3791/62418-v • 08:11 min • August 7th, 2021
August 7th, 2021 •
1, 1, 1,2
1Structural Genomics Consortium, University of Toronto, 2Department of Pharmacology and Toxicology, University of Toronto

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Diese Protokolle liefern die Methodik zur Beurteilung der enzymatischen Aktivität einzelner Mitglieder der Protein-Arginin-Methyltransferase-Familie (PRMT) in Zellen. Detaillierte Richtlinien zur Bewertung der PRMT-Aktivität unter Verwendung endogener und exogener Biomarker, Methyl-Arginin-erkennender Antikörper und Inhibitor-Werkzeugverbindungen werden beschrieben.

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Biologie Ausgabe 174
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