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Kvantitative metoder til undersøgelse af protein arginin methyltransferase 1-9 aktivitet i celler
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Kvantitative metoder til undersøgelse af protein arginin methyltransferase 1-9 aktivitet i celler

Article DOI: 10.3791/62418-v • 08:11 min • August 7th, 2021
August 7th, 2021 •
1, 1, 1,2
1Structural Genomics Consortium, University of Toronto, 2Department of Pharmacology and Toxicology, University of Toronto

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Disse protokoller indeholder den metode, der anvendes til at vurdere den enzymatiske aktivitet hos individuelle medlemmer af proteinalgininmethylentransferasefamilien (PRMT) i celler. Der er beskrevet detaljerede retningslinjer for vurdering af PRMT-aktivitet ved hjælp af endogene og eksogene biomarkører, methylalginin, der genkender antistoffer, og hæmmerværktøjsforbindelser.

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Biologi udgave 174
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