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Journal / Biology / Ca2+過渡現象とL型カルシウム電流の同時測定のための高品質マウス心房および心室筋細胞の分離…
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Biology

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JoVE Journal Biology
Ca2+過渡現象とL型カルシウム電流の同時測定のための高品質マウス心房および心室筋細胞の分離
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Ca2+過渡現象とL型カルシウム電流の同時測定のための高品質マウス心房および心室筋細胞の分離

Article DOI: 10.3791/61964-v • 06:22 min • November 3rd, 2020
November 3rd, 2020 •
*1,2,3, *1,2,3, 1,2, *1,2,3, *4,5,6
1Department of Medicine I, University Hospital Munich, Campus Großhadern, Ludwig-Maximilians University Munich (LMU), 2Partner Site Munich, Munich Heart Alliance (MHA), DZHK (German Centre for Cardiovascular Research), 3Walter Brendel Center of Experimental Medicine, Ludwig-Maximilians University Munich (LMU), 4Institute of Pharmacology and Toxicology, University Medical Center Göttingen, 5Partner Site Göttingen, DZHK (German Centre for Cardiovascular Research), 6Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), University of Göttingen
* These authors contributed equally

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マウスモデルは、不整脈形成の重要なメカニズムを研究することを可能にする。そのためには、パッチクランプ測定を行うために高品質の心筋細胞が必要です。ここでは、カルシウム一過性物質とL型カルシウム電流を同時に測定できる逆行性酵素ベースのランゲンドルフ灌流を介してマウス心房筋細胞と心室筋細胞を分離する方法について説明します。

Tags

今月のJoVE、第165号、不整脈、マウス筋細胞単離、ランゲンドルフ灌流、L型カルシウム電流、カルシウム一過性、パッチクランプ
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