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JoVE Journal Biology
Generering af hiPSC-afledte tarmorganoider til udviklings- og sygdomsmodelleringsapplikationer
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Generering af hiPSC-afledte tarmorganoider til udviklings- og sygdomsmodelleringsapplikationer

Article DOI: 10.3791/61199-v • 06:34 min • March 8th, 2024
March 8th, 2024 •
*1, *1, 1, 1, 1, 1
1Biodiscovery Institute, Translational Medical Sciences, School of Medicine, University of Nottingham
* These authors contributed equally

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Denne protokol tillader differentiering af humane pluripotente celler i intestinale organoider. Protokollen efterligner normal menneskelig udvikling ved at differentiere celler til en population af endelig endoderm, hindgut endoderm og derefter tarmepitel. Dette gør protokollen velegnet til at studere både tarmudvikling såvel som sygdomsmodelleringsapplikationer.

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Denne måned i JoVE udgave 205 tarmorganoider bagtarm stamceller endoderm betændelse sygdomsmodellering
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