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Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications
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Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

Article DOI: 10.3791/61199-v • 06:34 min • March 8th, 2024
March 8th, 2024 •
*1, *1, 1, 1, 1, 1
1Biodiscovery Institute, Translational Medical Sciences, School of Medicine, University of Nottingham
* These authors contributed equally

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Summary
Automatic Translation

This protocol allows for the differentiation of human pluripotent cells into intestinal organoids. The protocol mimics normal human development by differentiating cells into a population of definitive endoderm, hindgut endoderm and then intestinal epithelium. This makes the protocol suitable for studying both intestinal development as well as disease modelling applications.

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intestinal organoids hindgut stem cells endoderm inflammation disease modelling
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