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Kultur av Piglet Intestinal 3D Organoids fra kryopreserverte epitelkrypter og etablering av cellemonolayers
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Kultur av Piglet Intestinal 3D Organoids fra kryopreserverte epitelkrypter og etablering av cellemonolayers

Article DOI: 10.3791/64917-v • 08:19 min • February 10th, 2023
February 10th, 2023 •
1,2, 1, 3, 2, 4, 1, 1
1GenPhySE, Université de Toulouse, INRAE, ENVT, 2Lallemand Animal Nutrition, 3Institut NuMeCan, INRAE, INSERM, Université de Rennes, 4FG 16: Mycotic and Parasitic Agents and Mycobacteria, Robert Koch-Institute

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Her beskriver vi en protokoll for å dyrke gris intestinal 3D-organoider fra kryopreserverte epitelkrypter. Vi beskriver også en metode for å etablere cellemonolag avledet fra 3D-organoider, noe som gir tilgang til den apikale siden av epitelceller.

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