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Immunology and Infection

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JoVE Journal Immunology and Infection
Analyse van T-celreceptor-geïnduceerde calciuminstroom in primaire muizen T-cellen door full spectrum flow cytometrie
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Analyse van T-celreceptor-geïnduceerde calciuminstroom in primaire muizen T-cellen door full spectrum flow cytometrie

Article DOI: 10.3791/64526-v • 10:01 min • December 16th, 2022
December 16th, 2022 •
1, 1, 1
1Department of Immunology and Microbiology, University of Colorado-Anschutz School of Medicine

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Calciuminflux, een maat voor T-celsignalering, is een effectieve manier om reacties op T-celreceptorstimulatie te analyseren. Dit protocol voor het multiplexen van Indo-1 met panelen van antilichamen gericht op celoppervlakmoleculen maakt gebruik van de zeer flexibele mogelijkheden van volledige spectrum flowcytometrie.

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Immunologie en infectie Nummer 190
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