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Developmental Biology

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JoVE Journal Developmental Biology
Multiphotonen-Laserablation von zytoplasmatischen Mikrotubuli-Organisationszentren in Maus-Eizellen
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Multiphotonen-Laserablation von zytoplasmatischen Mikrotubuli-Organisationszentren in Maus-Eizellen

Article DOI: 10.3791/64439-v • 08:24 min • November 11th, 2022
November 11th, 2022 •
1,2, 3, 1
1Animal Sciences Research Center, University of Missouri, 2Universidad Nacional de Colombia, 3Advanced Light Microscopy Core, University of Missouri

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Es wird ein optimiertes Protokoll vorgestellt, das die Depletion zytoplasmatischer Mikrotubuli-Organisationszentren in Maus-Eizellen während der Metaphase I mit einem Nahinfrarot-Femtosekundenlaser ermöglicht.

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Developmental Biology Ausgabe 189 Eizelle metaphastische zytoplasmatische MTOC Meiose Laserablation
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