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Journal / Genetics / Entwicklung von Knock-out-Muskelzelllinien…
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JoVE Journal Genetics
Entwicklung von Knock-out-Muskelzelllinien mittels Lentivirus-vermittelter CRISPR/Cas9-Gen-Editing
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
Journal (Genetics)
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
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High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology
 
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Entwicklung von Knock-out-Muskelzelllinien mittels Lentivirus-vermittelter CRISPR/Cas9-Gen-Editing

Article DOI: 10.3791/64114-v • 10:12 min • June 16th, 2022
June 16th, 2022 •
1, 1, 1, 2, 2, 1, 1
1University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, 2Genome Center, University of California, Davis

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Das Protokoll beschreibt, wie Knock-out-Myoblasten mit CRISPR/Cas9 erzeugt werden können, beginnend mit dem Design von Guide-RNAs bis hin zur zellulären Klonierung und Charakterisierung der Knock-out-Klone.

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Genetik Ausgabe 184
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