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Biochemistry

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JoVE Journal Biochemistry
Um método semi-automatizado e reprodutível de base biológica para quantificar a deposição de cálcio in vitro
Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes
Journal (Biochemistry)
Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes
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Journal (Biochemistry)
Cloning of Solute Carriers (SLCs) into pHTBV1.1 Bacmid for High-Throughput Protein Expression (Video) | JoVE
 
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Um método semi-automatizado e reprodutível de base biológica para quantificar a deposição de cálcio in vitro

Article DOI: 10.3791/64029-v • 11:30 min • June 2nd, 2022
June 2nd, 2022 •
*1, *1, 1, 2, 1, 2, 1,3
1Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, 2Helmholtz Institute for Biomedical Engineering, Biointerface Group, RWTH Aachen University, 3Institute of Experimental Medicine and Systems Biology, RWTH Aachen University
* These authors contributed equally

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A doença cardiovascular é a principal causa de morte em todo o mundo. A calcificação vascular contribui substancialmente para a carga de morbidade e mortalidade cardiovascular. Este protocolo descreve um método simples para quantificar a precipitação de cálcio mediada por células musculares lisas vasculares in vitro por imagem fluorescente.

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Bioquímica Edição 184
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