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Journal / Biology / Cryo-elektron tomografi Fjerndataindsamling og…
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Cryo-elektron tomografi Fjerndataindsamling og subtomogram gennemsnit
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Cryo-elektron tomografi Fjerndataindsamling og subtomogram gennemsnit

Article DOI: 10.3791/63923-v • 08:55 min • July 12th, 2022
July 12th, 2022 •
1, 1, *1, *1,2,3
1Electron Bio-Imaging Centre, Diamond Light Source Ltd, Harwell Science & Innovation Campus, 2Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, 3Chinese Academy of Medical Sciences Oxford Institute, University of Oxford
* These authors contributed equally

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Den nuværende protokol beskriver højopløsnings kryo-elektrontomografi fjerndataindsamling ved hjælp af Tomo5 og efterfølgende databehandling og subtomogram gennemsnit ved hjælp af emClarity. Apoferritin bruges som et eksempel til at illustrere detaljerede trin-for-trin processer for at opnå en cryo-ET-struktur ved 2,86 Å opløsning.

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