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JoVE Journal Bioengineering
Isolatie, uitbreiding en differentiatie van mesenchymale stamcellen uit de infrapatellaire vetpad van de geitenstikkingsverbinding
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Isolatie, uitbreiding en differentiatie van mesenchymale stamcellen uit de infrapatellaire vetpad van de geitenstikkingsverbinding

Article DOI: 10.3791/63617-v • 12:06 min • August 2nd, 2022
August 2nd, 2022 •
*1,2, *1,2, 1,3, 1,2
1Department of Biological Sciences and Bioengineering, Indian Institute of Technology-Kanpur, 2The Mehta Family Centre for Engineering in Medicine, Indian Institute of Technology-Kanpur, 3Mechanobiology Institute, National University of Singapore
* These authors contributed equally

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Infrapatellaire vetkussen mesenchymale stamcellen (IFP-MSC's) kunnen gemakkelijk worden geïsoleerd uit de infrapatellaire vetschijf van het kniegewricht. Ze vermenigvuldigen zich goed in vitro, vormen CFU-F-kolonies en differentiëren in adipogene, chondrogene en osteogene afstammingslijnen. Hierin wordt de methodologie gegeven voor de isolatie, uitbreiding en differentiatie van IFP-MSC's van geitenstikselgewricht.

Tags

Bio-engineering Goat infrapatellar fat pad (IFP) mesenchymale stamcellen (MSC's) celisolatie celkweek multilineage potentieel regeneratieve geneeskunde tissue engineering
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