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Journal / Immunology and Infection / 回転細胞培養システムを用いた模擬微小重力下でのリンパ球の培養…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
回転細胞培養システムを用いた模擬微小重力下でのリンパ球の培養
Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells
Journal (Immunology and Infection)
Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells
Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis
Journal (Immunology and Infection)
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回転細胞培養システムを用いた模擬微小重力下でのリンパ球の培養

Article DOI: 10.3791/63296-v • 09:28 min • August 25th, 2022
August 25th, 2022 •
1,2, 1,2,4, 1,3
1Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, 2Krembil Research Institute, Toronto Western Hospital, University Health Network, 3Pathology and Lab Medicine, Hematopathology, Mount Sinai Hospital, Sinai Health Systems, 4Medical Oncology and Hematology, Princess Margaret Cancer Centre

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市販の回転細胞培養システムを用いて、専用の使い捨て培養容器を用いて模擬微小重力下でリンパ球を培養するためのステップバイステップガイドです。この培養方法は、いずれの浮遊型細胞培養にも適用することができる。

Tags

リトラクション、186号、浮遊細胞培養、リンパ球、模擬微小重力、時間平均重力ベクトル無効化、2Dクリノスタット、回転細胞培養システム、回転壁器装置
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