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Journal / Biochemistry / Harpiksassisteret indfangning kombineret med…
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Biochemistry

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JoVE Journal Biochemistry
Harpiksassisteret indfangning kombineret med isobarisk tandemmassemærkemærkning til multiplekset kvantificering af protein thioloxidation
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Harpiksassisteret indfangning kombineret med isobarisk tandemmassemærkemærkning til multiplekset kvantificering af protein thioloxidation

Article DOI: 10.3791/62671-v • 07:16 min • June 21st, 2021
June 21st, 2021 •
1, 1, 2, 1
1Integrative Omics, Biological Sciences Division, Pacific Northwest National Laboratory, 2Bioproducts Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University

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Protein thiol oxidation har betydelige konsekvenser under normale fysiologiske og patofysiologiske forhold. Vi beskriver detaljerne i en kvantitativ redoxproteomics-metode, der anvender harpiksassisteret indfangning, isobarisk mærkning og massespektrometri, hvilket muliggør stedsspecifik identifikation og kvantificering af reversibelt oxiderede cysteinrester af proteiner.

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Biokemi udgave 172 RAC TMT thiol redox proteomics cystein PTM støkiometri redox modifikationer
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