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JoVE Journal Biology
In vitro Tredimensionel spireanalyse af angiogenese ved hjælp af museembryonale stamceller til vaskulær sygdomsmodellering og lægemiddeltest
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In vitro Tredimensionel spireanalyse af angiogenese ved hjælp af museembryonale stamceller til vaskulær sygdomsmodellering og lægemiddeltest

Article DOI: 10.3791/62554-v • 08:04 min • May 11th, 2021
May 11th, 2021 •
1, 1, 1, 1,2,3
1Einthoven Laboratory for Experimental Vascular Medicine, Department of Internal Medicine (Nephrology), Leiden University Medical Center, 2Institute Physics for Medicine Paris, INSERM U1273, ESPCI Paris, CNRS FRE 2031, 3MEMOLIFE Laboratory of Excellence and PSL Research University

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Dette assay anvender embryonale stamceller fra mus, der er differentieret i embryoide kroppe dyrket i 3D-kollagengel, til at analysere de biologiske processer, der styrer spirende angiogenese in vitro. Teknikken kan anvendes til test af lægemidler, modellering af sygdomme og til at studere specifikke gener i forbindelse med deletioner, der er embryonalt dødelige.

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Denne måned i JoVE udgave 171 embryonale stamceller fra mus spirende angiogenese endotelcellespecifikation fartøjsmodning lægemiddeltestning sygdomsmodellering
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