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Journal / Developmental Biology / En forenklet metode for å generere nyreorganoider …
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JoVE Journal Developmental Biology
En forenklet metode for å generere nyreorganoider fra humane pluripotente stamceller
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En forenklet metode for å generere nyreorganoider fra humane pluripotente stamceller

Article DOI: 10.3791/62452-v • 07:39 min • April 13th, 2021
April 13th, 2021 •
1, 1, 2, 2, 2, 1,3
1Department of Developmental Biology, University of Pittsburgh, School of Medicine, 2Department of Molecular Medicine and Pathology, School of Medical Sciences, University of Auckland, 3Center for Critical Care Nephrology, University of Pittsburgh, School of Medicine

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Her beskriver vi en protokoll for å generere nyreorganoider fra humane pluripotente stamceller (hPSCer). Denne protokollen genererer nyreorganoider innen to uker. De resulterende nyreorganoidene kan dyrkes i store spinnerflasker eller multi-brønn magnetiske røreplater for parallelle legemiddeltestingsmetoder.

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Utviklingsbiologi Utgave 170 nyreorganoider CHIR99021 utslagsserumerstatning embryoide legemer nyrevev pluripotente stamceller suspensjonskultur
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