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Journal / Biology / Kryostrukturert belysning mikroskopisk…
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Biology

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Kryostrukturert belysning mikroskopisk datainnsamling fra kryogenisk bevarte celler
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Kryostrukturert belysning mikroskopisk datainnsamling fra kryogenisk bevarte celler

Article DOI: 10.3791/62274-v • 11:55 min • May 28th, 2021
May 28th, 2021 •
*1, *1, 1, 1, 1, 1,2, 1, 1, 3, 3, 4, 1
1Harwell Science and Innovation Campus, Beamline B24, Diamond Light Source, 2Division of Virology, Department of Pathology, University of Cambridge, 3ALBA Synchrotron, Beamline 09 - MISTRAL, 4Micron Advanced Imaging Consortium, Department of Biochemistry, University of Oxford
* These authors contributed equally

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Denne protokollen demonstrerer hvordan man kan avbilde biologiske kryokonserverte prøver ved hjelp av kryostrukturert belysningsmikroskopi. Vi demonstrerer metodikken ved å avbilde cytoskjelettet til U2OS-celler.

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Biologi utgave 171 kryogene avbildninger fluorescensmikroskopi 3D-avbildning mikroskopi med superoppløsning kryoSIM strukturert belysningsmikroskopi korrelativ avbildning
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