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Journal / Developmental Biology / Generering af inducerede pluripotente stamceller…
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Developmental Biology
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JoVE Journal Developmental Biology
Generering af inducerede pluripotente stamceller fra Turner Syndrom (45XO) Fosterceller til downstream modellering af neurologiske underskud forbundet med syndromet
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Generering af inducerede pluripotente stamceller fra Turner Syndrom (45XO) Fosterceller til downstream modellering af neurologiske underskud forbundet med syndromet

Article DOI: 10.3791/62240-v • 09:39 min • December 4th, 2021
December 4th, 2021 •
*1, *1, 2, 1, 1
1Manipal Institute of Regenerative Medicine, Manipal Academy of Higher Education, Manipal, Karnataka, India-576104, 2Department of Medical Genetics, Manipal Hospitals
* These authors contributed equally

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Denne protokol beskriver generering af integrationsfrie iPSC'er fra føtal væv fibroblaster gennem levering af episomal plasmid ved nucleofection efterfulgt af beskrivelse af metoder, der anvendes til iPSC-karakterisering og neuronal differentiering.

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Udviklingsbiologi Udgave 178 Omprogrammering Human induceret pluripotente stamceller Aneuploidies Medfødte misdannelser spontan fosterdød Turner Syndrom Neurologiske Underskud
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