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Serial Block-Face Scanning Electron Microscopie (SBF-SEM) van biologische weefselmonsters
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Serial Block-Face Scanning Electron Microscopie (SBF-SEM) van biologische weefselmonsters

Article DOI: 10.3791/62045-v • 09:21 min • March 26th, 2021
March 26th, 2021 •
1, 1, 1, 2, 2, 1, 3,4, 1,4
1College of Optometry, University of Houston, 2Department of Biology, DePauw University, 3Center for Translational Research on Inflammatory Diseases (CTRID), Michael E. DeBakey Veterans Affairs Medical Center, 4Children’s Nutrition Center, Baylor College of Medicine

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Dit protocol schetst een routinemethode voor het gebruik van seriële blok-gezicht scanning elektronenmicroscopie (SBF-SEM), een krachtige 3D-beeldvormingstechniek. Succesvolle toepassing van SBF-SEM hangt af van de juiste fixatie- en weefselkleuringstechnieken, evenals zorgvuldige overweging van beeldvormingsinstellingen. Dit protocol bevat praktische overwegingen voor het gehele proces.

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Biologie Serial Block-Face Scanning Electron Microscopy SBF-SEM 3D-EM 3D-Reconstruction Tissue Preparation Specimen Preparation Serial Sections Ultrastructure High-resolution Volumetric Imaging Biological Large Volume
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