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En tre-dimensionel model af sfæroider til undersøgelse tyktarmskræft stamceller
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En tre-dimensionel model af sfæroider til undersøgelse tyktarmskræft stamceller

Article DOI: 10.3791/61783-v • 06:38 min • January 22nd, 2021
January 22nd, 2021 •
1,2, 1,2, 1, 1,2
1Département de la recherche, Centre de Recherche en Cancérologie de Lyon, INSERM U1052, CNRS UMR5286, Université de Lyon, Université Lyon 1, Centre Léon Bérard, 2UMR-S1113 - IRFAC INSERM, Université de Strasbourg

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Denne protokol præsenterer en ny, robust, og reproducerbare kultur system til at generere og vokse tre-dimensionelle sfæroider fra Caco2 kolon adenocarcinoma celler. Resultaterne giver den første proof-of-concept for hensigtsmæssigheden af denne tilgang til undersøgelse af kræft stamcellebiologi, herunder reaktionen på kemoterapi.

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Kræftforskning Caco2 celler Kræft stamceller Cellespredning Tyktarmskræft Kolosfærer Kolosfærer vækst
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