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Journal / Biology / En enda cell dissociation strategi för molekylär…
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Biology

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JoVE Journal Biology
En enda cell dissociation strategi för molekylär analys av urinblåsan i musen efter ryggmärgsskada
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En enda cell dissociation strategi för molekylär analys av urinblåsan i musen efter ryggmärgsskada

Article DOI: 10.3791/61455-v • 09:50 min • June 17th, 2020
June 17th, 2020 •
*1,2, *1,2, 1, 3,4,5, 1,6, 1,2, 2,7, 2,7, 1,2
1Department of Urology, Boston Children's Hospital, 2Department of Surgery, Harvard Medical School, 3Division of Hematology/Oncology, Harvard Medical School Boston, 4Dana-Farber Cancer Institute, 5Broad Institute, 6Biological Biomedical Sciences Program, Division of Medical Sciences, Harvard Medical School, 7Vascular Biology Program, Boston Children's Hospital
* These authors contributed equally

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Målet med detta protokoll är att tillämpa ett optimerat vävnadsdisociationsprotokoll på en musmodell av ryggmärgsskada och validera metoden för encellsanalys genom flödescytometri.

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Biologi Nummer 160 Ryggmärg skada encellig cell dissociation flödescytometri murin
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