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Isolering av humane ventrikulære kardiomyocytter fra vibratom-cut myokardskiver
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Isolering av humane ventrikulære kardiomyocytter fra vibratom-cut myokardskiver

Article DOI: 10.3791/61167-v • 11:19 min • May 10th, 2020
May 10th, 2020 •
1, 1,2, 1,2
1Institute of Cellular and Molecular Physiology, Friedrich-Alexander University Erlangen-Nürnberg, 2Muscle Research Center Erlangen (MURCE), Friedrich-Alexander-Universität Erlangen-Nürnberg

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Presentert er en protokoll for isolering av menneskelige og animalske ventrikulære kardiomyocytter fra vibratom-cut myokardskiver. Høye utbytter av kalsiumtolerante celler (opptil 200 celler/mg) kan oppnås fra små mengder vev (<50 mg). Protokollen gjelder for myokardium utsatt for kald iskemi i opptil 36 timer.

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Biologi Utgave 159 human hjertesvikt myokardskiver kardiomyocyttisolering vibratom kontraktile myocytter kalsiumavbildning hjerteeksitasjonskobling
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