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JoVE Journal
Biochemistry

This content is Open Access.

I Vivo Hydroxyl Radical Protein Footprinting for studiet af proteininteraktioner i Caenorhabditis elegans
 
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I Vivo Hydroxyl Radical Protein Footprinting for studiet af proteininteraktioner i Caenorhabditis elegans

Article DOI: 10.3791/60910-v 08:00 min April 1st, 2020
April 1st, 2020

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

In vivo hurtig fotokemisk oxidation af proteiner (IV-FPOP) er en hydroxyl radikal protein fodspor teknik, der giver mulighed for kortlægning af proteinstruktur i deres oprindelige miljø. Denne protokol beskriver samling og opsætning af IV-FPOP mikrofluidic flow system.

Tags

Biokemi Caenorhabditis elegans hydroxyl radikalprotein fodaftryk FPOP massespektrometri in vivo proteomics
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