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Journal / Cancer Research / En roman Stromal Fibroblast-Moduleret 3D Tumor…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
En roman Stromal Fibroblast-Moduleret 3D Tumor Spheroid Model for at studere Tumor-Stroma Interaktion og Drug Discovery
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En roman Stromal Fibroblast-Moduleret 3D Tumor Spheroid Model for at studere Tumor-Stroma Interaktion og Drug Discovery

Article DOI: 10.3791/60660-v • 07:20 min • February 28th, 2020
February 28th, 2020 •
1, 1, 1, 1, 2, 1
1Department of Surgery, University of Miami School of Medicine, 2Analytical Imaging Core Facility, University of Miami School of Medicine

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En ny tre-dimensionel sfænode model baseret på den heterotypic interaktion af tumorceller og stromale fibroblaster er etableret. Her præsenterer vi coculture af tumorceller og stromale fibroblaster, time-lapse imaging, og konfokal mikroskopi til at visualisere dannelsen af sfæller. Denne tre-dimensionelle model tilbyder en relevant platform til at studere tumor-stroma interaktioner og teste kræft terapeutiske.

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Cancer Research 3D tumor sfærisk model tumor sfænoider multicellulære 3D sfæroider tumor stamceller melanom indlede celler (MIC) kræft-associerede fibroblaster (CAF) tumor stromale fibroblaster tumor-stromale interaktion tumor mikromiljø (TME)
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