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Indurre Meningococcal Meningitis Serogroup C in Topi tramite consegna intracisternale

Article DOI: 10.3791/60047-v • 10:03 min • November 5th, 2019
November 5th, 2019 •
1, 1, 1, 1, 1, 1, 2, 1, 1,3
1Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 2Department of Science and Technology, Sannio University, 3CEINGE – Advanced Biotechnology

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Qui, descriviamo un metodo per indurre la meningite meningococcica attraverso una via intracisternale di infezione nei topi adulti. Vi presentiamo un protocollo passo dopo passo dell'infezione da meningococco dalla preparazione dell'inoculum all'infezione intracisternale; quindi registrare la sopravvivenza animale e valutare i carichi batterici nei tessuti murini.

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Immunologia e infezione Numero 153 Infezione Neisseria meningitidis meningite meningococcica modelli murini iniezione intra-cisternale tessuto cerebrale ceppo mutante isogenico.
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