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Journal / Immunology and Infection / 利用 Cas9 Ribonucleoproteins 生成人 NK…
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Immunology and Infection

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JoVE Journal Immunology and Infection
利用 Cas9 Ribonucleoproteins 生成人 NK 细胞的研究
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利用 Cas9 Ribonucleoproteins 生成人 NK 细胞的研究

Article DOI: 10.3791/58237-v • 07:26 min • June 14th, 2018
June 14th, 2018 •
1, 2, 1, 3, 1, 1, 1, 1, 1, 1, 1, 3, 1
1Center for Childhood Cancer and Blood Disease, Nationwide Children's Hospital, 2Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine, University of Oxford, 3Center for Clinical and Translational Research, Nationwide Children's Hospital Research Institute

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在这里, 我们提出了一个协议, 基因改造主要或扩大人类自然杀手 (NK) 细胞使用Cas9 Ribonucleoproteins (Cas9/RNPs)。通过使用该协议, 我们生成的人 NK 细胞缺乏转化生长 factor–b 受体 2 (TGFBR2) 和嘌呤 phosphoribosyltransferase 1 (HPRT1)。

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免疫学和感染 问题 136 人原发性 nk 细胞 人扩张 nk 细胞 CRISPR/Cas9 Cas9/RNPs gRNAs TGFBR2 TGFβ抗 NK 细胞
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