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Biochemistry

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JoVE Journal Biochemistry

LERLIC-MS / MS进行深入表征和谷氨酰胺的定量和天冬酰胺脱酰胺在鸟枪蛋白质组学

Cryo-EM and Single-Particle Analysis with Scipion

Journal (Biochemistry)

Cryo-EM and Single-Particle Analysis with Scipion

Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET

Journal (Biochemistry)

Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET

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LERLIC-MS / MS进行深入表征和谷氨酰胺的定量和天冬酰胺脱酰胺在鸟枪蛋白质组学

Article DOI: 10.3791/55626-v • 08:01 min • April 9th, 2017

April 9th, 2017 •

Xavier Gallart-Palau*¹, Aida Serra*¹, Siu Kwan Sze¹

¹Division of Structural Biology and Biochemistry, School of Biological Sciences, Nanyang Technological University

* These authors contributed equally

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这里，我们提出的长条静电排斥亲水相互作用色谱 - 串联质谱（LERLIC-MS / MS）的方法的一个步骤一步协议。这是一种新的方法，使通过鸟枪蛋白质组学的谷氨酰胺和天冬酰胺脱酰胺亚型的第一次定量和定性。

Tags

生物化学，第122，脱酰胺，谷氨酰胺，天冬酰胺，ERLIC，LERLIC，退行性蛋白修饰DPMS，翻译后修饰翻译后修饰，酰氨，长度长的阴离子交换LAX，猎枪蛋白质组学，HILIC。

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