Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / In Vitro Colony analyser för att karakterisera…
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Developmental Biology
In Vitro Colony analyser för att karakterisera Tri-potenta stamceller isolerade från vuxen Murina bukspottkörtel
Thawing, Culturing, and Cryopreserving Drosophila Cell Lines
Journal (Developmental Biology)
Thawing, Culturing, and Cryopreserving Drosophila Cell Lines
Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids
Journal (Developmental Biology)
Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids
 
Click here for the English version

In Vitro Colony analyser för att karakterisera Tri-potenta stamceller isolerade från vuxen Murina bukspottkörtel

Article DOI: 10.3791/54016-v • 09:31 min • June 10th, 2016
June 10th, 2016 •
*1,2, *1, 1, 1, 1, 1, 1, 3, 1
1Diabetes and Metabolism Research Institute, Beckman Research Institute of City of Hope, 2Irell & Manella Graduate School of Biological Sciences, Beckman Research Institute of City of Hope, 3Division of Chemistry and Chemical Engineering, California Institute of Technology
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

In vitro kolonianalyser för att upptäcka självförnyelse och differentiering av stamceller som isolerats från vuxna murina bukspottkörteln utformas. I dessa analyser, pankreas stamceller ger upphov till cellkolonier i tre-dimensionell rymd i metylcellulosa innehållande halvfast medium. Protokoll för hantering av enskilda celler och karakterisering av enskilda kolonier beskrivs.

Tags

Developmental Biology pankreatiska kolonibildande enheter progenitorceller fluorescensaktiverad cellsortering (FACS) enda koloni analyser enkelcellanalyser extracellulära matrisproteiner laminin hydrogel metylcellulosa halvfast medium hela montering immunofärgning mikroflödes PCR vuxna möss
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter