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En strategi för att validera roll Callose-medierad Plasmodesmal Gating i Tropic Response
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En strategi för att validera roll Callose-medierad Plasmodesmal Gating i Tropic Response

Article DOI: 10.3791/53513-v • 12:18 min • April 17th, 2016
April 17th, 2016 •
*1, *1, 1, 1, 2, 1
1Division of Applied Life Science (BK21 plus program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 2Biotechnology Research Institute, Chinese Academy of Agricultural Sciences
* These authors contributed equally

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Den här artikeln beskriver metoder för screening av gener som kontrollerar plasmodesmal permeabilitet och därmed auxin gradient under tropiska svar. Detta inkluderar mätningen av graden av tropic respons i hypokotyl av Arabidopsis thaliana och kontroll plasmodesmal permeabilitet med 8-hydroxipyren-1,3,6-trisulfonsyra (HPTS) lastning och bedömning slutligen callose nivå.

Tags

Cellbiologi , Plasmodesmata (PD) callose auxin tropism hypokotyl symplasmic rörelse anilin blå 8-hydroxipyren-1,3,6-trisulfonsyra (HPTS) mutant
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