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A Step-by-step Metodo per la ricostituzione di un Transporter ABC in particelle lipidiche Nanodisc

Article DOI: 10.3791/3910-v • 12:25 min • August 31st, 2012
August 31st, 2012 •
1, 1, 1
1Department of Biochemistry and Molecular Biology, University of British Columbia

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Nanodiscs sono piccole particelle discoidali che incorporano proteine ​​di membrana in una piccola zona di doppio strato di fosfolipidi. Forniamo un protocollo visivo che mostra il passo-passo incorporazione del trasportatore MalFGK2 in un disco.

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Numero 66 Nanodiscs proteine lipidi di membrana trasportatori ABC trasportatori maltosio MalFGK
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