Assessing Serum Neutralization Against SARS-CoV-2 with Pseudotyped Viruses

November 21st, 2023

Studying Host-Virus Interactions with Pseudotyped Viruses (Video) | JoVE

01:03 min

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SARS-CoV-2 Pseudotype Virus Production and Serum Neutralization Assay (Video) | JoVE

04:50 min

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Tobia Fantoni*¹, Michele Bissoli*¹, Chiara Stefani¹, Mauro Voi¹, Alexandrina Dabija¹, Rebecca Casula¹, Domenico Luca Minafra¹, Julys da Fonseca Palmeira², Enrique Roberto Argañaraz², Martin Mayora-Neto³, Nigel J. Temperton³, Donato Zipeto¹, Alessandra Ruggiero¹

¹Department of Neuroscience, Biomedicine and Movement, University of Verona, ²Laboratory of Molecular Neurovirology, Faculty of Health Science, University of Brasília, ³Viral Pseudotype Unit, Medway School of Pharmacy, Universities of Kent and Greenwich at Medway

* These authors contributed equally

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Les virus pseudotypés (PV) sont des virions défectueux de réplication qui sont utilisés pour étudier les interactions hôte-virus dans des conditions plus sûres que la manipulation de virus authentiques. Nous présentons ici un protocole détaillé qui montre comment les PV du SRAS-CoV-2 peuvent être utilisés pour tester la capacité de neutralisation du sérum des patients après la vaccination contre la COVID-19.

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Assessing Serum Neutralization Against SARS-CoV-2 with Pseudotyped Viruses

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