Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Gene Knock-in door CRISPR/Cas9 en celsortering in …
JoVE Journal
Immunology and Infection
Author Produced

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Immunology and Infection
Gene Knock-in door CRISPR/Cas9 en celsortering in macrofaag- en T-cellijnen
Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria
Journal (Immunology and Infection)
Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria
Infecting Mice with Malassezia spp. to Study the Fungus-Host Interaction
Journal (Immunology and Infection)
Infecting Mice with Malassezia spp. to Study the Fungus-Host Interaction
 
Click here for the English version

Gene Knock-in door CRISPR/Cas9 en celsortering in macrofaag- en T-cellijnen

Article DOI: 10.3791/62328-v • 11:32 min • November 13th, 2021
November 13th, 2021 •
1, 1, 1, 1, 1, 1, 1, 1, 2, 1
1The Laboratory of Genetic Regulators in the Immune System, School of Laboratory Medicine, Xinxiang Medical University, 2Centre d’Immunologie de Marseille-Luminy, Aix Marseille Université

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Dit protocol maakt gebruik van fluorescerende reporters en celsortering om knock-in experimenten in macrofaag- en T-cellijnen te vereenvoudigen. Twee plasmiden worden gebruikt voor deze vereenvoudigde knock-in experimenten, namelijk een CRISPR/Cas9- en DsRed2-tot expressie brengend plasmide en een homoloog recombinatiedonorplasmide dat EBFP2 tot expressie brengt, dat permanent is geïntegreerd op de Rosa26-locus in immuuncellen.

Tags

Immunologie en infectie knock-in CRISPR/Cas9 Rosa26 macrofaag T-cel hACE2
Read Article
Waiting X
Simple Hit Counter