JoVE Journal
Genetics
Genetics
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
High resolution melting analysis (HRM) is a sensitive and rapid solution for genetic variant detection. It depends on sequence differences that result in heteroduplexes changing the shape of the melting curve. By combing HRM and agarose gel electrophoresis, different types of genetic variants such as indels can be identified.