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En Patient-Afledt Xenograft Model for Venøs misdannelser
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En Patient-Afledt Xenograft Model for Venøs misdannelser

Article DOI: 10.3791/61501-v • 06:51 min • June 15th, 2020
June 15th, 2020 •
*1, *1, 1,2
1Division of Experimental Hematology and Cancer Biology, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine
* These authors contributed equally

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Vi præsenterer en detaljeret protokol til at generere en murine xenograft model af venøs misdannelser. Denne model er baseret på subkutan injektion af patientbaserede endotelceller, der indeholder hyperaktiverende TIE2- og/eller PIK3CA-genmutationer. Xenograft læsioner nøje opsummere de histopatologiske træk ved VM patientvæv.

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Udviklingsbiologi Venøs misdannelser xenograft endotelceller TIE2 PIK3CA vaskulær læsion
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