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Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
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Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Article DOI: 10.3791/52925-v • 06:56 min • September 24th, 2015
September 24th, 2015 •
1, 2
1Broad Institute, 2Department of Biological Engineering, Massachusetts Institute of Technology

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Summary
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We describe an endpoint digital assay for quantifying nucleic acids with a simplified (analog) readout. We measure bulk fluorescence of droplet-based digital assays using a standard qPCR machine rather than specialized instrumentation and confirm our results by microscopy.

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Bulk Readout Digital Nucleic Acid Quantification Assays Digital Assays Rare Cells Detection Nucleic Acid Molecules Counting Conventional Real-time PCR Instrument Fluorescence Measurement Droplet-based Digital Assays Synthetic Droplet Mixtures Droplet Digital Multiple Displacement Amplification (MDA) Assay Quantitative MDA Digital Reaction Format Digital PCR Speed Up Assay Readout Partitioned Assays Advantages Specialized Readout Instrumentation Dynamic Range Limitations Standard Sample Requirement Quantitative Whole Genome Amplification (WGA) Contaminants Detection
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