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Glycan Profiling of Plant Cell Wall Polymers using Microarrays
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Glycan Profiling of Plant Cell Wall Polymers using Microarrays

Article DOI: 10.3791/4238-v • 12:30 min • December 17th, 2012
December 17th, 2012 •
1,2, 3, 1, 1, 4, 1,2
1Australian Centre of Excellence in Plant Cell Walls, School of Botany, University of Melbourne, 2Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3CSIRO Plant Industry, Black Mountain Laboratories, 4Department of Plant Biology and Biotechnology, University of Copenhagen

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Summary
Automatic Translation

A technique called Comprehensive Microarray Polymer Profiling (CoMPP) for the characterisation of plant cell wall glycans is described. This method combines the specificity of monoclonal antibodies directed to defined glycan-epitopes with a miniature microarray analytical platform allowing screening of glycan occurrence in a broad range of biological contexts.

Tags

Glycan Profiling Plant Cell Wall Polymers Microarrays Heterogeneous Glycans Biosynthesis Function Glycosyl Residues Linkages Ring Structure Isomeric Configuration Anomeric Configuration Non-sugar Residues Cell And Tissue Types Development Environmental Cues Genes Arabidopsis Reverse Genetics Approaches
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