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In vitro Reconstitution of the Active T. castaneum Telomerase
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In vitro Reconstitution of the Active T. castaneum Telomerase

Article DOI: 10.3791/2799-v • 09:26 min • July 14th, 2011
July 14th, 2011 •
1, 1, 1
1Gene Expression and Regulation, The Wistar Institute, University of Pennsylvania

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Summary
Automatic Translation

Efforts to isolate the catalytic subunit of telomerase, TERT, in sufficient quantities for structural studies, have been met with limited success for more than a decade. Here, we present methods for the isolation of the recombinant Tribolium castaneum TERT (TcTERT) and the reconstitution of the active T. castaneum telomerase ribonucleoprotein (RNP) complex in vitro.

Tags

Telomerase T. Castaneum Catalytic Subunit Isolation Recombinant Telomerase Ribonucleoprotein In Vitro Reconstitution DNA Repeats Telomeres Reverse Transcriptase Linear Chromosomes End-to-end Fusion Degradation DNA Replication Hayflick Limit Somatic Cells Cancer Cells Cell Immortality Protein Subunit Integral RNA Component Template Synthesis Crystal Structure
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