Instituto Nacional de Perinatologia 4 articles published in JoVE Medicine A Modified Sonographic Algorithm for Image Acquisition in Life-Threatening Emergencies in the Critically Ill Newborn Daniel Ibarra-Ríos1, Eunice Valeria Serpa-Maldonado1, José Guadalupe Mantilla-Uresti1, Rogelio Guillén-Torres1, Naomi Aguilar-Martínez2, Alejandra Sánchez-Cruz3, Deneb Algedi Morales-Barquet4, Rosario Becerra-Becerra5, Horacio Márquez-González6 1Hemodynamic Consultation and Ultrasound in the Critically Ill Neonate, Hospital Infantil de México Federico Gómez (HIMFG), 2Neonatology Department, HIMFG, 3Neonatal Intensive Care Unit, Hospital de Ginecología y Obstetricia Número 4, Luis Castelazo Ayala, Instituto Mexicano del Seguro Social, 4Head of Intermediate Care, Instituto Nacional de Perinatología, 5Head of the Echocardiography Lab, HIMFG, 6Clinical Investigation Department, HIMFG Here, we present a protocol that can be applied in the neonatal intensive care unit and the delivery room in relation to three scenarios: cardiac arrest, hemodynamic deterioration, or respiratory decompensation. This protocol can be performed with a state-of-the-art ultrasound machine or an affordable handheld device; an image acquisition protocol is carefully detailed. Medicine Isolation of Viable Adipocytes and Stromal Vascular Fraction from Human Visceral Adipose Tissue Suitable for RNA Analysis and Macrophage Phenotyping Guadalupe Estrada-Gutierrez1, Eyerahi Bravo-Flores2, Veronica Ortega-Castillo3, Veronica Flores-Rueda4, Ismael Mancilla-Herrera5, Salvador Espino Y Sosa6, Maribel Sánchez-Martínez2, Otilia Perichart-Perera7, Mario Solis-Paredes8 1Research Division, Instituto Nacional de Perinatologia, 2Department of Immunobiochemistry, Instituto Nacional de Perinatologia, 3Department of Obstetrics, Instituto Nacional de Perinatologia, 4Department of Academic Programs and Continuing Education, Instituto Nacional de Perinatologia, 5Department of Infectology and Immunology, Instituto Nacional de Perinatologia, 6Clinical Research Branch, Instituto Nacional de Perinatologia, 7Department of Nutrition and Bioprogramming, Instituto Nacional de Perinatologia, 8Department of Human Genetics and Genomics, Instituto Nacional de Perinatologia, Mexico City, Mexico This protocol provides an efficient collagenase digestion method for isolation of viable adipocytes and stromal vascular fraction-SVF cells from human visceral fat in a single process, including methodology to obtain high-quality RNA from adipocytes and phenotypification of SVF-macrophages through staining of multiple membrane-bound markers for analysis by flow cytometry. Neuroscience Culture of Neurospheres Derived from the Neurogenic Niches in Adult Prairie Voles Daniela Ávila-González1, Larry J. Young2, Francisco Camacho1, Raúl G. Paredes1,3, Néstor F. Díaz4, Wendy Portillo1 1Departamento de Neurobiología Conductual y Cognitiva, Instituto de Neurobiología, Universidad Nacional Autónoma de México, 2Silvio O. Conte Center for Oxytocin and Social Cognition, Center for Translational Social Neuroscience, Department of Psychiatry and Behavioral Sciences, Yerkes National Primate Research Center, Emory University, 3Escuela Nacional de Estudios Superiores Juriquilla, Universidad Nacional Autónoma de México, 4Departamento de Fisiología y Desarrollo Celular, Instituto Nacional de Perinatología We established the conditions to culture neural progenitor cells from the subventricular zone and dentate gyrus of the adult brain of prairie voles, as a complementary in vitro study, to analyze the sex-dependent differences between neurogenic niches that could be part of functional plastic changes associated with social behaviors. Developmental Biology In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane Daniela Avila-González1, Guadalupe García-López1, Néstor E. Díaz-Martínez2, Héctor Flores-Herrera3, Anayansi Molina-Hernández1, Wendy Portillo4, Néstor F. Díaz1 1Departamento de Fisiología y Desarrollo Celular, Instituto Nacional de Perinatología, 2Biotecnología Médica y Farmacéutica CONACYT, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), 3Departamento de Inmunobioquímica, Instituto Nacional de Perinatología, 4Instituto de Neurobiología, UNAM This protocol describes the isolation of epithelial cells from different anatomical regions of the human amniotic membrane to determine their heterogeneity and functional properties for possible application in clinical and physiopathological models.
